Coding
[trbCf]

Part:BBa_J23014:Design

Designed by: Will Bosworth   Group: iGEM06_Berkeley   (2006-07-24)


trbCf open reading frame basic part


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal NgoMIV site found at 587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Actually, oligos JL14 and JL15 did not work, so we had to create 20mers JL16 and JL17 in order to get the trbCf gene and will work with that.


Source

PCR JL14/JL15 on Olambda (679bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)

References